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chicken anti ß gal  (Danaher Inc)


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    Danaher Inc chicken anti ß gal
    (A-C) Wing discs of hs-Flp ; ds Cr+:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (A) , hs-Flp ; d sCr Δ A:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (B), or hs-Flp ; ds Cr Δ D:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (C) , with mitotic clones homozygous for ds Cr+:V5 (A), d sCr Δ A:V5 (B), or ds Cr Δ D:V5 (C), marked by loss <t>of</t> <t>ß-gal</t> (red), outlined with white dotted lines, to show effects on Dachs:GFP (green). (D-F) Wing discs of hs-Flp ; ds Cr+:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (D) , hs-Flp ; ds Cr Δ A:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (E) , or hs-Flp ; ds Cr Δ D:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (F) stained for Wg (white) and E-cad (red) and with Flip-out clones expressing Dachs:GFP (green/white). Panels at right show Dachs:GFP (white) in higher magnification images of the boxed regions. Proximal (P) to distal (D) orientation is indicated. (G) Histogram showing classification of Dachs polarity in Flp-out clones in different ds genotypes. 60 clones were scored per genotype. (H) Western blot showing results of co-immunoprecipitation experiments between V5-tagged Ds proteins and FLAG-tagged Dachs transfected into S2 cells. Top three panels show blots on total cell lysates transfected to express the indicated proteins, using the antibodies indicated at right. Bottom two panels show blots on proteins immunoprecipitated with anti-V5 beads and detected with V5 or FLAG antibodies.
    Chicken Anti ß Gal, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chicken anti ß gal/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    chicken anti ß gal - by Bioz Stars, 2026-06
    86/100 stars

    Images

    1) Product Images from "Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling"

    Article Title: Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling

    Journal: bioRxiv

    doi: 10.1101/2024.04.03.587940

    (A-C) Wing discs of hs-Flp ; ds Cr+:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (A) , hs-Flp ; d sCr Δ A:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (B), or hs-Flp ; ds Cr Δ D:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (C) , with mitotic clones homozygous for ds Cr+:V5 (A), d sCr Δ A:V5 (B), or ds Cr Δ D:V5 (C), marked by loss of ß-gal (red), outlined with white dotted lines, to show effects on Dachs:GFP (green). (D-F) Wing discs of hs-Flp ; ds Cr+:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (D) , hs-Flp ; ds Cr Δ A:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (E) , or hs-Flp ; ds Cr Δ D:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (F) stained for Wg (white) and E-cad (red) and with Flip-out clones expressing Dachs:GFP (green/white). Panels at right show Dachs:GFP (white) in higher magnification images of the boxed regions. Proximal (P) to distal (D) orientation is indicated. (G) Histogram showing classification of Dachs polarity in Flp-out clones in different ds genotypes. 60 clones were scored per genotype. (H) Western blot showing results of co-immunoprecipitation experiments between V5-tagged Ds proteins and FLAG-tagged Dachs transfected into S2 cells. Top three panels show blots on total cell lysates transfected to express the indicated proteins, using the antibodies indicated at right. Bottom two panels show blots on proteins immunoprecipitated with anti-V5 beads and detected with V5 or FLAG antibodies.
    Figure Legend Snippet: (A-C) Wing discs of hs-Flp ; ds Cr+:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (A) , hs-Flp ; d sCr Δ A:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (B), or hs-Flp ; ds Cr Δ D:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (C) , with mitotic clones homozygous for ds Cr+:V5 (A), d sCr Δ A:V5 (B), or ds Cr Δ D:V5 (C), marked by loss of ß-gal (red), outlined with white dotted lines, to show effects on Dachs:GFP (green). (D-F) Wing discs of hs-Flp ; ds Cr+:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (D) , hs-Flp ; ds Cr Δ A:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (E) , or hs-Flp ; ds Cr Δ D:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (F) stained for Wg (white) and E-cad (red) and with Flip-out clones expressing Dachs:GFP (green/white). Panels at right show Dachs:GFP (white) in higher magnification images of the boxed regions. Proximal (P) to distal (D) orientation is indicated. (G) Histogram showing classification of Dachs polarity in Flp-out clones in different ds genotypes. 60 clones were scored per genotype. (H) Western blot showing results of co-immunoprecipitation experiments between V5-tagged Ds proteins and FLAG-tagged Dachs transfected into S2 cells. Top three panels show blots on total cell lysates transfected to express the indicated proteins, using the antibodies indicated at right. Bottom two panels show blots on proteins immunoprecipitated with anti-V5 beads and detected with V5 or FLAG antibodies.

    Techniques Used: Clone Assay, Staining, Expressing, Western Blot, Immunoprecipitation, Transfection

    (A-F) Wing discs from homozygous ds CrD Δ II:V5 (A) or ds CrD Δ III:V5 (D) stained for Ds:V5. Red square boxes in (A) show approximate locations in the hinge and wing pouch (WP) corresponding to the higher magnification panels at right. ( A,D ) Lower magnification images showing the wing pouch and hinge regions, Scale bar = 100 µm. (B,C,E,F) Higher magnification images depicting part of the wing hinge ( B,E ) or proximal wing pouch ( C,F ) in discs expressing V5-tagged Ds from ds CrD Δ II:V5 ( B,C ) or ds CrD Δ III:V5 ( E,F ). Scale bar = 10 µm. (G,H) Wing discs from hs-Flp ; ds CrD Δ II:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (G) , or hs-Flp ; ds CrD Δ III:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (H), with mitotic clones homozygous for ds CrD Δ II:V5 (G), or ds CrD Δ III:V5 (H), marked by loss of ß-gal (red), outlined with white dotted lines, to show effects on Dachs:GFP (green). (I,J) Wing discs from hs-Flp ; ds CrD Δ II:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (I) , or hs-Flp ; ds CrD Δ III:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (J) , stained for expression of Wg (white) and E-cad (red) and with Flip-out clones expressing Dachs:GFP (green/white). Panels at right show Dachs:GFP (white) in higher magnification images of the boxed regions. Proximal (P) to distal (D) orientation is indicated. (K) Histogram showing the classification of Dachs polarity in Flp-out clones in different ds genotypes as normal (blue color) or mispolarised (orange). N= number scored.
    Figure Legend Snippet: (A-F) Wing discs from homozygous ds CrD Δ II:V5 (A) or ds CrD Δ III:V5 (D) stained for Ds:V5. Red square boxes in (A) show approximate locations in the hinge and wing pouch (WP) corresponding to the higher magnification panels at right. ( A,D ) Lower magnification images showing the wing pouch and hinge regions, Scale bar = 100 µm. (B,C,E,F) Higher magnification images depicting part of the wing hinge ( B,E ) or proximal wing pouch ( C,F ) in discs expressing V5-tagged Ds from ds CrD Δ II:V5 ( B,C ) or ds CrD Δ III:V5 ( E,F ). Scale bar = 10 µm. (G,H) Wing discs from hs-Flp ; ds CrD Δ II:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (G) , or hs-Flp ; ds CrD Δ III:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (H), with mitotic clones homozygous for ds CrD Δ II:V5 (G), or ds CrD Δ III:V5 (H), marked by loss of ß-gal (red), outlined with white dotted lines, to show effects on Dachs:GFP (green). (I,J) Wing discs from hs-Flp ; ds CrD Δ II:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (I) , or hs-Flp ; ds CrD Δ III:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (J) , stained for expression of Wg (white) and E-cad (red) and with Flip-out clones expressing Dachs:GFP (green/white). Panels at right show Dachs:GFP (white) in higher magnification images of the boxed regions. Proximal (P) to distal (D) orientation is indicated. (K) Histogram showing the classification of Dachs polarity in Flp-out clones in different ds genotypes as normal (blue color) or mispolarised (orange). N= number scored.

    Techniques Used: Staining, Expressing, Clone Assay



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    (A-C) Wing discs of hs-Flp ; ds Cr+:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (A) , hs-Flp ; d sCr Δ A:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (B), or hs-Flp ; ds Cr Δ D:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (C) , with mitotic clones homozygous for ds Cr+:V5 (A), d sCr Δ A:V5 (B), or ds Cr Δ D:V5 (C), marked by loss <t>of</t> <t>ß-gal</t> (red), outlined with white dotted lines, to show effects on Dachs:GFP (green). (D-F) Wing discs of hs-Flp ; ds Cr+:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (D) , hs-Flp ; ds Cr Δ A:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (E) , or hs-Flp ; ds Cr Δ D:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (F) stained for Wg (white) and E-cad (red) and with Flip-out clones expressing Dachs:GFP (green/white). Panels at right show Dachs:GFP (white) in higher magnification images of the boxed regions. Proximal (P) to distal (D) orientation is indicated. (G) Histogram showing classification of Dachs polarity in Flp-out clones in different ds genotypes. 60 clones were scored per genotype. (H) Western blot showing results of co-immunoprecipitation experiments between V5-tagged Ds proteins and FLAG-tagged Dachs transfected into S2 cells. Top three panels show blots on total cell lysates transfected to express the indicated proteins, using the antibodies indicated at right. Bottom two panels show blots on proteins immunoprecipitated with anti-V5 beads and detected with V5 or FLAG antibodies.
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    Image Search Results


    (A-C) Wing discs of hs-Flp ; ds Cr+:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (A) , hs-Flp ; d sCr Δ A:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (B), or hs-Flp ; ds Cr Δ D:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (C) , with mitotic clones homozygous for ds Cr+:V5 (A), d sCr Δ A:V5 (B), or ds Cr Δ D:V5 (C), marked by loss of ß-gal (red), outlined with white dotted lines, to show effects on Dachs:GFP (green). (D-F) Wing discs of hs-Flp ; ds Cr+:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (D) , hs-Flp ; ds Cr Δ A:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (E) , or hs-Flp ; ds Cr Δ D:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (F) stained for Wg (white) and E-cad (red) and with Flip-out clones expressing Dachs:GFP (green/white). Panels at right show Dachs:GFP (white) in higher magnification images of the boxed regions. Proximal (P) to distal (D) orientation is indicated. (G) Histogram showing classification of Dachs polarity in Flp-out clones in different ds genotypes. 60 clones were scored per genotype. (H) Western blot showing results of co-immunoprecipitation experiments between V5-tagged Ds proteins and FLAG-tagged Dachs transfected into S2 cells. Top three panels show blots on total cell lysates transfected to express the indicated proteins, using the antibodies indicated at right. Bottom two panels show blots on proteins immunoprecipitated with anti-V5 beads and detected with V5 or FLAG antibodies.

    Journal: bioRxiv

    Article Title: Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling

    doi: 10.1101/2024.04.03.587940

    Figure Lengend Snippet: (A-C) Wing discs of hs-Flp ; ds Cr+:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (A) , hs-Flp ; d sCr Δ A:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (B), or hs-Flp ; ds Cr Δ D:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (C) , with mitotic clones homozygous for ds Cr+:V5 (A), d sCr Δ A:V5 (B), or ds Cr Δ D:V5 (C), marked by loss of ß-gal (red), outlined with white dotted lines, to show effects on Dachs:GFP (green). (D-F) Wing discs of hs-Flp ; ds Cr+:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (D) , hs-Flp ; ds Cr Δ A:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (E) , or hs-Flp ; ds Cr Δ D:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (F) stained for Wg (white) and E-cad (red) and with Flip-out clones expressing Dachs:GFP (green/white). Panels at right show Dachs:GFP (white) in higher magnification images of the boxed regions. Proximal (P) to distal (D) orientation is indicated. (G) Histogram showing classification of Dachs polarity in Flp-out clones in different ds genotypes. 60 clones were scored per genotype. (H) Western blot showing results of co-immunoprecipitation experiments between V5-tagged Ds proteins and FLAG-tagged Dachs transfected into S2 cells. Top three panels show blots on total cell lysates transfected to express the indicated proteins, using the antibodies indicated at right. Bottom two panels show blots on proteins immunoprecipitated with anti-V5 beads and detected with V5 or FLAG antibodies.

    Article Snippet: Primary antibodies were used for staining were: Mouse anti-V5 (Invitrogen R960-25; preabsorbed at 1:10 dilution, then used at 1:50), Rat anti-E-cadherin (DSHB DCAD2-c 1:200), Chicken anti-ß-gal (Abcam ab9361; 1:100), Rat anti-Fat (1:1500) , Mouse anti-Wg (DSHB 9A4; 1:300), Rabbit anti-Dcr2 (Abcam ab4732, 1:1600), Rabbit anti-FLAG (Sigma F3165; 1:200).

    Techniques: Clone Assay, Staining, Expressing, Western Blot, Immunoprecipitation, Transfection

    (A-F) Wing discs from homozygous ds CrD Δ II:V5 (A) or ds CrD Δ III:V5 (D) stained for Ds:V5. Red square boxes in (A) show approximate locations in the hinge and wing pouch (WP) corresponding to the higher magnification panels at right. ( A,D ) Lower magnification images showing the wing pouch and hinge regions, Scale bar = 100 µm. (B,C,E,F) Higher magnification images depicting part of the wing hinge ( B,E ) or proximal wing pouch ( C,F ) in discs expressing V5-tagged Ds from ds CrD Δ II:V5 ( B,C ) or ds CrD Δ III:V5 ( E,F ). Scale bar = 10 µm. (G,H) Wing discs from hs-Flp ; ds CrD Δ II:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (G) , or hs-Flp ; ds CrD Δ III:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (H), with mitotic clones homozygous for ds CrD Δ II:V5 (G), or ds CrD Δ III:V5 (H), marked by loss of ß-gal (red), outlined with white dotted lines, to show effects on Dachs:GFP (green). (I,J) Wing discs from hs-Flp ; ds CrD Δ II:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (I) , or hs-Flp ; ds CrD Δ III:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (J) , stained for expression of Wg (white) and E-cad (red) and with Flip-out clones expressing Dachs:GFP (green/white). Panels at right show Dachs:GFP (white) in higher magnification images of the boxed regions. Proximal (P) to distal (D) orientation is indicated. (K) Histogram showing the classification of Dachs polarity in Flp-out clones in different ds genotypes as normal (blue color) or mispolarised (orange). N= number scored.

    Journal: bioRxiv

    Article Title: Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling

    doi: 10.1101/2024.04.03.587940

    Figure Lengend Snippet: (A-F) Wing discs from homozygous ds CrD Δ II:V5 (A) or ds CrD Δ III:V5 (D) stained for Ds:V5. Red square boxes in (A) show approximate locations in the hinge and wing pouch (WP) corresponding to the higher magnification panels at right. ( A,D ) Lower magnification images showing the wing pouch and hinge regions, Scale bar = 100 µm. (B,C,E,F) Higher magnification images depicting part of the wing hinge ( B,E ) or proximal wing pouch ( C,F ) in discs expressing V5-tagged Ds from ds CrD Δ II:V5 ( B,C ) or ds CrD Δ III:V5 ( E,F ). Scale bar = 10 µm. (G,H) Wing discs from hs-Flp ; ds CrD Δ II:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (G) , or hs-Flp ; ds CrD Δ III:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (H), with mitotic clones homozygous for ds CrD Δ II:V5 (G), or ds CrD Δ III:V5 (H), marked by loss of ß-gal (red), outlined with white dotted lines, to show effects on Dachs:GFP (green). (I,J) Wing discs from hs-Flp ; ds CrD Δ II:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (I) , or hs-Flp ; ds CrD Δ III:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (J) , stained for expression of Wg (white) and E-cad (red) and with Flip-out clones expressing Dachs:GFP (green/white). Panels at right show Dachs:GFP (white) in higher magnification images of the boxed regions. Proximal (P) to distal (D) orientation is indicated. (K) Histogram showing the classification of Dachs polarity in Flp-out clones in different ds genotypes as normal (blue color) or mispolarised (orange). N= number scored.

    Article Snippet: Primary antibodies were used for staining were: Mouse anti-V5 (Invitrogen R960-25; preabsorbed at 1:10 dilution, then used at 1:50), Rat anti-E-cadherin (DSHB DCAD2-c 1:200), Chicken anti-ß-gal (Abcam ab9361; 1:100), Rat anti-Fat (1:1500) , Mouse anti-Wg (DSHB 9A4; 1:300), Rabbit anti-Dcr2 (Abcam ab4732, 1:1600), Rabbit anti-FLAG (Sigma F3165; 1:200).

    Techniques: Staining, Expressing, Clone Assay

    Journal: eLife

    Article Title: Retinoic acid signaling mediates peripheral cone photoreceptor survival in a mouse model of retina degeneration

    doi: 10.7554/eLife.76389

    Figure Lengend Snippet:

    Article Snippet: Antibody , (Chicken polyclonal) anti-B-galactosidase , Aves Labs , BGL1010 , (1:3000).

    Techniques: Recombinant, Expressing, Plasmid Preparation, Sequencing, RNAscope